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. Author manuscript; available in PMC: 2019 May 29.
Published in final edited form as: Science. 2018 Apr 6;360(6384):99–102. doi: 10.1126/science.aan8795

Fig. 1. qNSCs arrest in G0 or G2.

Fig. 1

(A) qNSCs are smaller than proliferating NSCs and extend a primary process, which is retracted upon activation from quiescence. Proliferating NSCs in the embryo do not exhibit a primary process prior to entering quiescence.

(B) 73±0.79% of qNSCs (green) are CycA+ (red). n=10 tVNCs, ~150 NSCs each.

(C) Cell cycle phase assessment using FUCCI and pH3.

(D) Percentages of NSCs (outlined) in each cell cycle phase during quiescence. Colours as in (C). n=5 tVNCs, ~150 NSCs each. ?: undetermined.

(E) DAPI intensities of CycA+ and CycA- qNSC nuclei are significantly different (p=2.20x10-16, Kolgomorov-Smirnov test). n=10 tVNCs, ~75 NSCs each. A.U.: arbitrary units. 1 A.U.: mean DAPI intensity of the CycA- population. Error bars indicate S.E.M.

(F) Features of G2 and G0 qNSCs.

Images are single section confocal images, unless indicated otherwise, and anterior is up in this and all subsequent figures.