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. Author manuscript; available in PMC: 2019 May 29.
Published in final edited form as: Science. 2018 Apr 6;360(6384):99–102. doi: 10.1126/science.aan8795

Fig. 4. Trbl induces and maintains quiescence through different mechanisms.

Fig. 4

(A) Top row: Cdc25String protein (green) expression in NSCs (red) before and during quiescence (percentages are mean±SEM). n=10 tVNCs/time point, ~130 NSCs each. Maximum intensity projections. Bottom row: In situ hybridisation against cdc25string mRNA at the same stages.

(B) Percentages of Cdc25String protein+ NSCs in control (trblEP3519 heterozygote) vs mutant tVNCs. n=10 tVNCs/genotype, ~110 NSCs each. ***: p=9.08x10-5, Kolgomorov-Smirnov test.

(C and D) Quantification of qNSCs in epistasis experiments between GFP-Trbl and AktACT (C) or PI3KACT (D). n>10 tVNCs/condition, ~80 NSCs each. ***: p<3.39x10-9. ns: p>0.05, one way ANOVA followed by post-hoc Tukey’s HSD test. In (D), there is no significant difference between GFP-Trbl alone and GFP-Trbl+PI3KACT.

(E) Three-step model for Trbl activity.

Red lines indicate medians.