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. 2019 May 28;10:2344. doi: 10.1038/s41467-019-09996-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — p43 is associated with the longitudinal muscle of the parasite and the intestinal mucus and the surrounding epithelial matrix of the host. a Western blot of cecal and colonic mucus samples from naive (N), Trichuris muris-infected (d35) mice, and T. muris whole excretome/secretome (E/S) probed with anti-p43 antibody. b Cecal sections from infected mice stained with anti-p43 and 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI) (merged), demonstrating staining within the worm (white asterisk) and extracellularly within the host intestinal lumen (red asterisk). c, d Transverse section of a worm taken from cecal sections from infected mice stained with anti-p43. d p43 staining within the worm (merged with bright field). e Transverse section of an adult T. muris hybridized with a p43 Cy5 probe (red) and myosin fluorescein isothiocyanate (FITC) probe (green); a merged image is shown, counterstained with DAPI. f, g Electron micrograph of adult T. muris stained with anti-p43 and colloidal gold particle-conjugated secondary antibodies. (Cut.= cuticle; LMF = longitudinal muscle fibers; white asterisk = within the worm; red asterisk = within the host intestinal lumen)