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. 2019 May 28;10(6):413. doi: 10.1038/s41419-019-1652-8

Fig. 2. FH expression and enzymatic activity were induced by CDDP and reduced CDDP cytotoxicity.

Fig. 2

a Western blot of FH expression in CDDP-treated SGC7901 cells with FH knockdown. b FH enzymatic activity in cells treated with or without CDDP after FH knockdown (**P < 0.01, Student’s t-test). Data are presented as the mean ± s.d. (n = 3). c IC50 of CDDP in SGC7901 cells with FH knockdown. d An annexin-V/PI assay detected the apoptosis of FH knockdown and control cells treated with CDDP (**P < 0.01, Student’s t-test). Data are presented as the mean ± s.d. (n = 3). e Representative images (left) and quantification (right) of immunofluorescence staining of c-PARP in SGC7901 and HGC27 cells treated with CDDP after FH knockdown (scale bar: 100 μm, **P < 0.01, Student’s t-test). Data are presented as the mean ± s.d. (n = 3). f CDDP and/or exogenous fumarate (5 mM) were utilized to the GC cell lines with FH knockdown or control. MTS assay determined the viability of the indicated groups (72 h, *P < 0.05, Student’s t-test). Data are presented as the mean ± s.d. (n = 3). g FH and c-PARP expression in FH over-expression cells with or without CDDP treated