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. 2019 May 28;9:7964. doi: 10.1038/s41598-019-44399-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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FTD cytotoxicity in TK1-knock-out cells. (A) Schematic diagram of the TK1 locus on Chr17q25.3. Exons are denoted by black rectangles and introns are shown in light grey. (B) Experimental scheme of TK1 knock-out. Three PCR fragments, 600–700 base pairs of the right and left homology arms and a puromycin resistance cassette, were cloned into pBluescript SK+. (C) Western blot analysis of TK1 protein (left) and quantitative RT-PCR analysis of TK1 mRNA (right) in HCT 116 parental and TK1-knock-out cells. Expression of TK1 was normalised against that of β-actin and is plotted relative to that in HCT 116 parental cells. Data are means ± s.d. of three independent experiments. (D) Cell viability assay. Cells were treated with nine points of dilution series of FTD for 3 days and then their viability was determined. The viability of cells not treated with FTD was defined as 100%. Data are means ± s.d. of three independent experiments.