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. 2019 May 28;9:7964. doi: 10.1038/s41598-019-44399-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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AFMID expression in TK1-knock-out cells. (A) Schematic diagram around the TK1 locus on Chr17. (B–D) Expression of AFMID was determined by quantitative RT-PCR, normalised against that of β-actin and plotted relative to that in HCT 116 cells. (B) TK1-knock-out cell lines. (C) Cloned HCT 116 cell lines. (D) The HCT 116/TK1KO ex.4 cell line and its clones whose puromycin resistance cassettes were removed by the Cre-loxP recombination system. Data are means ± s.d. of three independent experiments.