Skip to main content
. 2019 May 22;9:398. doi: 10.3389/fonc.2019.00398

Figure 1.

Figure 1

Effect of circPCMTD1 on cells growth and proliferation. (A) After transfection of overexpressing vector, the relative expression of circPCMTD1 in U251 and U118MG was detected by qRT-PCR. GAPDH was used as control. Data represent means ± SD (n = 3, each group). (B) After transfection of circPCMTD1 siRNA and control siRNA, the knockdown efficiency of circPCMTD1 was confirmed by qRT-PCR. GAPDH was used as control. (C,D) CCK-8 assays showed that overexpressing circPCMTD1 in the U251 and U118MG cell line, the cell viability was upregulated. (E,F). CCK-8 assays showed that cell proliferation was suppressed when circPCMTD1was silenced. ###p < 0.001 or ***p < 0.001 vs. control vector group for si-circ, **P < 0.01 or ##p < 0.01 vs. control vector group for si-circ, *P < 0.05 or #p < 0.05 vs. control vector group for si-circ, # for si-circ-1 and * for si-circ-2, n = 4. (G,H). The cells were accelerated to S/G2/M phase when circPCMTD1 was upregulated. Conversely, U251 and U118MG cells with circPCMTD1 stably silencing exhibited a massive G1 phase arrest. ***p < 0.001 vs. control vector group for over-circ, ###p < 0.001 vs. control vector group for si-circ, n = 4.