Figure 3.

Macaque sequences are described in the legend for Fig. 1. The human variants (var 1 to 13) are from Kang et al.⁴⁷. Enzyme cleavage sites were predicted using a Prosper server⁵⁵. The underlined predicted cleavage sites are those observed in macaque proteins but not in human ones. The amino acid substitution observed at position 7 does not modify the MMP9 cleavage site. The presence of a threonine at position 20 of macaque protein (instead of a methionine as in other macaque variants and as in human and most nonhuman primates) destroys an elastase 2 cleavage site (rectangle). Six human polymorphic cleavage sites are absent from macaque proteins. Also note that the macaque proteins can have two caspase 1 cleavage sites instead of only one in human proteins (and rare macaque variants). The second caspase 1 cleavage site (rectangle) of macaque proteins is encoded by a duplicated 27 bp motif.