Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 28;9:7967. doi: 10.1038/s41598-019-44449-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Super-resolution microscopy reveals that LAMP1-positive vesicles directly associate with the PVM in HeLa cells as well as in primary murine hepatocytes. STED images of HeLa cells (a) and primary murine hepatocytes (b) infected with mCherry-expressing P. berghei (PbmCherry). Images were taken using the Leica TCS SP8 STED microscope. Scale bars are 10 µm. (a) HeLa cells were fixed and stained with α-UIS4 (green; Oregon Green 488) and α-LAMP1 (red; Alexa532). (b) Primary murine hepatocytes were fixed and stained with α-UIS4 (red; Alexa532) and α-LAMP1 (green; Oregon Green 488). Enlargements of the indicated boxed part represented next to each image show possible fusion of vesicles with the PVM. Furthermore, it shows that LAMP1 localizes in the PVM of the parasite. Note that the resolution in the primary murine hepatocytes is decreased compared to the HeLa cells. The cultivation of primary murine hepatocytes requires coating with collagen, which partially interferes with the imaging. Additionally, primary murine hepatocytes are relatively thick compared to HeLa cells, which also decreases the resolution.