Table 1.
Primer list.
| Name | Sequence (5′ ->3′) | Restriction site |
|---|---|---|
| c-ssu-fw | GTGTAGTAACATCAGTTATTGTGTG | |
| d-ssu-fw | ATACTGTATAACAGGTAAGCTGTTATTGTG | |
| pL0017-rev | TTTCCCAGTCACGACGTTG | |
| cd-ssu-rev | CTTAGTGTTTTGTATTAATGTCGATTTG | |
| mEos3.2-fw | GGATCCAATGAGTGCGATTAAGCC | BamHI |
| mEos3.2-rev | TCTAGATTATCGTCTGGCATTGTCAGG | XbaI |
| mClover3-fw | CGTCAGCAACCGGTATGGTGAGCAAGGGCGAGGAGCTGTTC | AgeI |
| mRuby3-rev | GAATTCCTTGTACAGCTCGTCCATG | EcoRI |
| SPofLAMP1-fw | CTCGAGATGGCGCCCCGCAGC | XhoI |
| SPofLAMP1-rev | ACCGGTTGCTGACGCACAATGCAT | AgeI |
| LAMP1ΔSP-fw | TGTACAAGGAATTCGCAATGTTTATG | EcoRI |
| LAMP1-rev | TCTAGATTAGATAGTCTGGTAGCCTGCG | XbaI |
| KillerRed-fw | TGGATCCATGGGTTCAGAG | BamHI |
| KillerRed-rev | TTCTAGATTAATCCTCGTCGCTACC | XbaI |
| LAMP1-GFP-fw | ATCGGACCGATGGCGCCCCGCAG | RsrII |
| LAMP1-GFP-rev | ATCGGTCCGTTACTTGTACAGCTCG | RsrII |
| mTurquoise2-fw | AT(GGATCC)ACCGGTCGCCACCATGGTGAGCAAGGGCGAG | BamHI |
| mTurquoise2-rev | AT(TCTAGA)TTACTTGTACAGCTCGTCCATG | XbaI |