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. 2019 May 28;10(6):414. doi: 10.1038/s41419-019-1635-9

Fig. 3. ZCCHC10 knockdown promotes proliferation, migration, and invasion of the normal lung epithelial cell line Beas-2b.

Fig. 3

a ZCCHC protein levels in the untreated cells (Mock) and the cells with stably expressing ZCCHC10 shRNAs (shZh10, shZh-2, shZh-3, and shZh-4) or scramble shRNA (shNC). b The cell viability was determined by MTT assay. c Representative images of colony (left panel) and the colony numbers (right panel). Bar length: 1 cm. d Representative images (left panel) of wound area at the indicated time and percentage of wound closure at 24 h after scratching (right panel). Bar length: 50 μm. e Representative images (left panel) and statistical analysis (right panel) of invaded cells in the Transwell invasion assay. Bar length: 50 μm. All the values were presented as means ± SD for three independent experiments. Differences between two groups were analyzed by student t-test; *p ≤ 0.05 and **p ≤ 0.01