Fig. 7. ZCCHC10 protein exerts tumor-suppressive function via p53.

a WB analysis of p53, p21, Bax, Bcl2, CCND1, cleaved Caspase 3, CDH1, MMP9, Slug, and Snail proteins following overexpression or knockdown of ZCCHC10. b, c WB analysis of the indicated proteins after treatment with pifithrin-α (15 μM) or Nutlin3 (10 μM) for 72 h. d, e Flow cytometric analysis of cell cycle (d) and apoptosis (e) after treatment with pifithrin-α (15 μM) or Nutlin3 (10 μM) for 72 h. Representative images and corresponding statistical plots were shown in upper and lower panel, respectively. All the values were presented as means ± SD for three independent experiments