Table 2.
Experimental design used in PBD study for the seven selected factors along with the experimentally obtained response values for cell-bound phytase production by W. saturnus NCIM 3298
| Run no. |
Factor | Phytase production (IU/g DWB) |
||||||
|---|---|---|---|---|---|---|---|---|
| Glucose (%) |
NH4NO3 (%) |
Yeast extract (%) |
Citric acid (%) |
Incubation period (h) |
FeSO4 (mM) |
PH | ||
| 1 | 10 | 0.2 | 0.5 | 2.0 | 16 | 3.0 | 7.0 | 45 ± 6.5 |
| 2 | 2.0 | 0.2 | 0.1 | 2.0 | 16 | 3.0 | 7.0 | 10 ± 1.3 |
| 3 | 10 | 0.2 | 0.1 | 0.2 | 36 | 0.5 | 7.0 | 211 ± 11.8 |
| 4 | 2.0 | 0.2 | 0.1 | 0.2 | 16 | 0.5 | 5.0 | 269 ± 6.4 |
| 5 | 10 | 2.0 | 0.1 | 0.2 | 16 | 3.0 | 5.0 | 219 ± 15.4 |
| 6 | 10 | 2.0 | 0.1 | 2.0 | 36 | 3.0 | 5.0 | 91 ± 4.8 |
| 7 | 2.0 | 2.0 | 0.5 | 2.0 | 16 | 0.5 | 5.0 | 72 ± 3.5 |
| 8 | 10 | 0.2 | 0.5 | 2.0 | 36 | 0.5 | 5.0 | 170 ± 4.0 |
| 9 | 2.0 | 2.0 | 0.5 | 0.2 | 36 | 3.0 | 7.0 | 116 ± 3.1 |
| 10 | 10 | 2.0 | 0.5 | 0.2 | 16 | 0.5 | 7.0 | 184 ± 12.1 |
| 11 | 2.0 | 0.2 | 0.5 | 0.2 | 36 | 3.0 | 5.0 | 123 ± 10.1 |
| 12 | 2.0 | 2.0 | 0.1 | 2.0 | 36 | 0.5 | 7.0 | 98 ± 6.5 |
The culture was grown at 30 °C as described in “Materials and methods”. The values given are the average of three independent experiments