Table 5.
Effect of phytase treatment during saccharification and fermentation on ethanol production and on DDGS phytate and free phosphate content
| Treatmenta | Reducing sugar concentration of liquefied mash (g/L) | Ethanol production (g/L) | Phytate content (mg/100 g) |
Free phosphate content (mg/100 g) |
|||
|---|---|---|---|---|---|---|---|
| Liquefied mash | Fermentation broth | Liquefied mash | DDGS | Liquefied mash | DDGS | ||
| Raw corn | 1074 ± 10.7 | 37.48 ± 1.9 | |||||
| A | 79.9 ± 1.1 | 12.8 ± 0.6 | 12.4 ± 0.1 | 357.7 ± 29.5 | 284.3 ± 16.7 | 314.4 ± 4.1 | 320.5 ± 8.9 |
| B | 72.7 ± 1.5 | 13.1 ± 0.2 | 10.5 ± 0.2 | 863.0 ± 10.8 | 816.7 ± 15.8 | 40.2 ± 1.2 | 54.4 ± 1.9 |
aSaccharification carried out in presence of A) Amylases + W. saturnus NCIM 3298 and B) Amylases only. Fermentation carried out using A) S. cerevisiae + W. saturnus NCIM 3298 and B) S. cerevisiae. p value < 0.05 (one-tailed t test); the values given are the average of three independent experiments