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. 2019 May 28;12:38. doi: 10.1186/s12284-019-0298-6

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 5 — OsMADS57 directly regulates the expression of OsGA2ox3 and EUI1 by binding to the promoter regions of OsGA2ox3 and EUI1, respectively. a Schematic diagrams of the effector and reporter constructs used in the dual-luciferase reporter assays. b and c The transient transactivation assay in rice protoplasts. d Schematic diagram of OsGA2ox3 and EUI1 promoter regions showing the CArG-box. e and f EMSA showed that OsMADS57 bound to CArG-box in the promoter regions of OsGA2ox3 (e) and EUI1 (f). Three independent experiments were conducted with similar results. Data are means± SD (n = 9). Error bars indicate SD