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. 2019 May 22;10:1127. doi: 10.3389/fimmu.2019.01127

Figure 2.

Figure 2

miR-152-3p promoted B-cell responsiveness. (A–C) Upregulation of miR-152-3p expression in the B-cells of healthy controls increased CD40, CD80, and CD86 expression levels. Transfected cells were stained with PE-Cy7-conjugated anti-human CD40, FITC-conjugated anti-human CD80, and PerCP-Cy5.5-conjugated anti-human CD86, and then were analyzed by using flow cytometry. (D–F) Downregulation of miR-152-3p expression in the B-cells of patients with SLE decreased the expression levels of CD40, CD80, and CD86. Transfected cells were stained with PE-Cy7-conjugated anti-human CD40, FITC-conjugated anti-human CD80, and PerCP-Cy5.5-conjugated anti-human CD86, and analyzed using flow cytometry. (G) The expression of miRNA-152-3p in different transfection groups. (H) Statistical analysis of percentage of CD40, CD80, and CD86 in different transfection groups. All experiments were performed in triplicate. The variables were compared using the paired t-test. *P < 0.05 and **P < 0.01.