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. 2019 May 22;10:1132. doi: 10.3389/fimmu.2019.01132

Figure 4.

Figure 4

TLR2 regulates the phagocytosis and phagosome maturation of NPCs. (A,B) GFP-labeled S. aureus were incubated with NPCs from wild-type (WT) and Tlr2−/− mice, after which the interaction was compared by flow cytometry analysis. MI% represents mean intensities percentage. *P < 0.05, the group of WT vs. the group of Tlr2−/−. (C,D) The mean intensities percentage (MI%) at 4°C (phagocytosis was inhibited at 4°C) and 37°C were analyzed, respectively. A significant difference in DMI% (MI%37°C-MI%4°C) was observed in NPCs from WT mice. *P < 0.05, the group of WT vs. the group of Tlr2−/−. (E) Phagolysosomes formation decreased significantly in Tlr2−/− NPCs compared with WT NPCs based on Lyso-Tracker Red staining. The scale bar represents 100 μm. P-values were determined by T-test and one-way ANOVA. All data are presented as the means ± S.D from three independent experiments.