FIG 5.
Fungal NF-κB-type velvet regulators are required for proper bapA expression and BaP degradation. (A) Levels of bapA mRNA were measured using 2−ΔΔCT relative to actA (γ-actin) (ΔCT) and then relative to the WT (ΔΔCT). Time points indicate hours after the switch to 0.1% glucose medium. Technical and biological triplicates were used for each time point and treatment. *, P < 0.05; **, P < 0.01. (B) Residual BaP remaining after being cultured for 7 days with each strain. Note the lack of BaP degradation in ΔveA and ΔvelB strains. Three separate experiments were performed in triplicate. *, P < 0.05; **, P < 0.01.