FIG 2.

The NlpE N-terminal domain is sufficient for tolerance of LolB depletion and activation of Cpx. (A) Schematic of NlpE structure in its extended conformation. The N-terminal domain (orange) is joined to the C-terminal domain (blue) via a linker region (black). Sites of truncations are marked with spheres; green spheres indicate truncations that are able to activate Cpx, red spheres indicate truncations that fail to activate Cpx, and gray spheres show the Cys residues in a putatively redox-sensitive CXXC motif. (B) nlpE mutants were tested for their ability to tolerate LolB depletion (− arabinose) in an lpp(ΔK58) ΔosmB background. (C) Relative LacZ levels in ΔnlpE cells harboring a PcpxP-lacZ transcriptional reporter and overproducing plasmid-borne NlpE variants targeted to the OM. (D) Relative LacZ levels in ΔnlpE cells encoding a PcpxP-lacZ transcriptional reporter and overproducing plasmid-borne NlpE(DD) variants targeted to the IM. Data are means ± standard deviations.