TABLE 1.
Summary of NlpE constructs in this study
| Name | Description | Membrane targeting | Cpx activation |
|---|---|---|---|
| NlpE | Full-length wild-type NlpE | OM trafficked | Yes |
| NlpE(DD) | Full-length NlpE with N2D and N3D substitutions that cause avoidance of LolCDE | IM retained | Yes |
| NlpE1–121 | NlpE that lacks the C-terminal domain | OM trafficked | Yes |
| NlpE(DD)1–121 | Lacks the C-terminal domain; has the Lol avoidance signal | IM retained | Yes |
| NlpE1–101 | Lacks the C-terminal domain and the linker region | OM trafficked | Yes |
| NlpE(DD)1–-101 | Lacks the C-terminal domain and the linker region; has the Lol avoidance signal | IM retained | Yes |
| NlpE1–94 | Lacks the C-terminal domain, the linker region, and a portion of the N-terminal domain | OM trafficked | No |
| NlpE(DD)1–94 | Lacks the C-terminal domain, the linker region, and a portion of the N-terminal domain; has the Lol avoidance signal | IM retained | No |
| NlpE1–82 | Lacks the C-terminal domain, the linker region, and a portion of the N-terminal domain | OM trafficked | No |
| NlpE(DD)1–82 | Lacks the C-terminal domain, the linker region, and a portion of the N-terminal domain; has the Lol avoidance signal | IM retained | No |
| NlpE(C31S C34S) | Substitutions in N-terminal domain Cys residues proposed to form a disulfide bond | OM trafficked | Yes |