Antibodies |
|
High Affinity Anti-HA-HRP (3F10) |
Roche |
RRID:AB_390914
|
Anti-GFP-HRP |
Miltenyi Biotec |
RRID:AB_2470
|
|
Bacterial Strains |
|
E.coli (DH5α) |
N/A |
N/A |
A. tumefaciens (AGL1) |
N/A |
N/A |
|
Chemicals, Peptides and Recombinant Proteins |
|
Murashige &Skoog (MS) medium |
Duchefa Biochemie |
M0222.0050 |
Silwet® L-77 |
Phytotech Labs |
S7777 |
Epibrassinolide |
Santa-Cruz Biotechnology |
sc-211419 |
Abscisic Acid |
Sigma Aldrich |
862169 |
Brassinazole |
Sigma Aldrich |
SML1406 |
Luciferin |
Promega |
E1601 |
X-glucuronide |
Sigma Aldrich |
B5285-10MG |
SuperSignal West Femto |
Thermo Fisher Scientific |
34095 |
SuperSignal West Pico PLUS |
Thermo Fisher Scientific |
34580 |
FastDigest SacI |
Thermo Fisher Scientific |
FD1133 |
FastDigest NotI |
Thermo Fisher Scientific |
FD0593 |
FastDigest EcoRI |
Thermo Fisher Scientific |
FD0274 |
FastDigest BamHI |
Thermo Fisher Scientific |
FD0054 |
FastDigest Green Buffer (10X) |
Thermo Fisher Scientific |
B72 |
|
Critical Commercial Assays |
|
RNeasy Mini Kit |
QIAGEN |
74106 |
RNase-Free DNase Set (50) |
QIAGEN |
79254 |
QIAprep Spin Miniprep Kit |
QIAGEN |
27106 |
Nucleospin Gel and PCR Clean-up |
Macherey-Nagel |
740609 |
RevertAid First Strand cDNA Synthesis Kit |
Thermo Fisher Scientific |
K1621 |
SsoAdvanced Universal SYBR Green Supermix |
Bio-Rad |
1725271 |
|
Deposited Data |
|
PIF4/5-HA western blots |
https://zenodo.org/record/1343501#.XH_f2ohKg2w |
N/A |
BES1-GFP western blots |
https://zenodo.org/record/1480822#.XH_fCohKg2w |
N/A |
GUS staining images |
https://zenodo.org/record/2022607#.XH_f74hKg2w |
N/A |
Raw data for figure generation |
https://zenodo.org/record/2592526#.XIk36yhKg2w |
N/A |
|
Experimental Organisms |
|
Col-0 |
N/A |
N/A |
Ws |
N/A |
N/A |
Ler
|
N/A |
N/A |
pif4-101 |
3 |
Garlic_114_G06 |
pif5 (pil6-1) |
43 |
SALK_087012 |
pif7-1 |
44 |
SALK_044061 |
pif4-101/pif5 (aka pil6-1) |
3 |
Garlic_114_G06/ SALK_087012 |
pif4-101/pif5 (aka pil6-1)/ pif7-1
|
45 |
Garlic_114_G06/ SALK_087012/ SALK_044061 |
abaQ (pyr1-1/pyl1-1/pyl2-1/pyl4-1) |
14 |
Point/ Salk_054640/ CSHL_GT2864_1/ Sail_517_C08 |
snrk2.2/snrk2.3
|
15 |
GABI_807G04/ Salk_107315 |
arebQ (areb1/ areb2/ abf3/ abf1-1) |
13 |
SALK_002984/ SALK_069523/ SALK_096965/SALK_132819 |
abi5-1 |
46 |
N/A |
bes1-D |
27 |
N/A |
gsk3-triple (bin2-3bil1-1bil2-1) |
47 |
FLAG_593C09/Wisonsin KO /Wisonsin KO |
bsk5 |
NASC (characterized in 34) |
SALK_051739C |
aba2-1 |
48 |
N/A |
aba3-1 |
48 |
N/A |
della global (gai-t6/ rga-t2/ rgl1-1/ rgl2-1/ rgl3-4) |
49 |
N/A |
pPIL1:LUC |
17 |
N/A |
pIAA19:LUC |
New line |
N/A |
pDR5V2:GUS |
New line |
N/A |
pDR5V2:GUS/ pif4-101/ pif5 (pil6-1) |
New line |
N/A /Garlic_114_G06/ SALK_087012 |
p35S:PIF4-HA |
Franklin Lab, Bristol, UK |
N/A |
p35S:PIF5-HA |
Franklin Lab, Bristol, UK |
N/A |
p35S:BES1-GFP |
28 |
N/A |
Tomato- S.lycopersicum var. “Moneymaker” |
Intratuin, NL |
EAN# 8717263349600 |
Tobacco- N. benthamiana |
N/A |
N/A |
|
Recombinant DNA |
|
pENTR_D-TOPO |
Thermo Fisher |
K2400-20 |
LucTrap3 |
N/A |
GenBank: AY968054.1
|
pGREENII0179 |
N/A |
N/A |
pUC57:DR5v2 |
Dolf Weijers (WUR) |
N/A |
|
Software and Algorithms |
|
Microsoft Excel 2010 |
Microsoft |
N/A |
Microsoft Powerpoint 2010 |
Microsoft |
N/A |
Microsoft Word 2010 |
Microsoft |
N/A |
SPSS Statistics 24 |
IBM |
N/A |
Snapgene |
GSL Biotech LLC |
N/A |
ImageJ |
NIH |
N/A |
ICY |
Quantitative Image Analysis Unit, Institut Pasteur |
N/A |
GIMP2 |
The GIMP Development Team |
N/A |
ViiA 7 Software |
Thermo Fisher Scientific |
N/A |
Prism 6 |
Graphpad |
N/A |