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. 2019 May 20;29(10):1669–1676.e4. doi: 10.1016/j.cub.2019.03.042
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

High Affinity Anti-HA-HRP (3F10) Roche RRID:AB_390914
Anti-GFP-HRP Miltenyi Biotec RRID:AB_2470

Bacterial Strains

E.coli (DH5α) N/A N/A
A. tumefaciens (AGL1) N/A N/A

Chemicals, Peptides and Recombinant Proteins

Murashige &Skoog (MS) medium Duchefa Biochemie M0222.0050
Silwet® L-77 Phytotech Labs S7777
Epibrassinolide Santa-Cruz Biotechnology sc-211419
Abscisic Acid Sigma Aldrich 862169
Brassinazole Sigma Aldrich SML1406
Luciferin Promega E1601
X-glucuronide Sigma Aldrich B5285-10MG
SuperSignal West Femto Thermo Fisher Scientific 34095
SuperSignal West Pico PLUS Thermo Fisher Scientific 34580
FastDigest SacI Thermo Fisher Scientific FD1133
FastDigest NotI Thermo Fisher Scientific FD0593
FastDigest EcoRI Thermo Fisher Scientific FD0274
FastDigest BamHI Thermo Fisher Scientific FD0054
FastDigest Green Buffer (10X) Thermo Fisher Scientific B72

Critical Commercial Assays

RNeasy Mini Kit QIAGEN 74106
RNase-Free DNase Set (50) QIAGEN 79254
QIAprep Spin Miniprep Kit QIAGEN 27106
Nucleospin Gel and PCR Clean-up Macherey-Nagel 740609
RevertAid First Strand cDNA Synthesis Kit Thermo Fisher Scientific K1621
SsoAdvanced Universal SYBR Green Supermix Bio-Rad 1725271

Deposited Data

PIF4/5-HA western blots https://zenodo.org/record/1343501#.XH_f2ohKg2w N/A
BES1-GFP western blots https://zenodo.org/record/1480822#.XH_fCohKg2w N/A
GUS staining images https://zenodo.org/record/2022607#.XH_f74hKg2w N/A
Raw data for figure generation https://zenodo.org/record/2592526#.XIk36yhKg2w N/A

Experimental Organisms

Col-0 N/A N/A
Ws N/A N/A
Ler N/A N/A
pif4-101 3 Garlic_114_G06
pif5 (pil6-1) 43 SALK_087012
pif7-1 44 SALK_044061
pif4-101/pif5 (aka pil6-1) 3 Garlic_114_G06/ SALK_087012
pif4-101/pif5 (aka pil6-1)/ pif7-1 45 Garlic_114_G06/ SALK_087012/ SALK_044061
abaQ (pyr1-1/pyl1-1/pyl2-1/pyl4-1) 14 Point/ Salk_054640/ CSHL_GT2864_1/ Sail_517_C08
snrk2.2/snrk2.3 15 GABI_807G04/ Salk_107315
arebQ (areb1/ areb2/ abf3/ abf1-1) 13 SALK_002984/ SALK_069523/ SALK_096965/SALK_132819
abi5-1 46 N/A
bes1-D 27 N/A
gsk3-triple (bin2-3bil1-1bil2-1) 47 FLAG_593C09/Wisonsin KO /Wisonsin KO
bsk5 NASC (characterized in 34) SALK_051739C
aba2-1 48 N/A
aba3-1 48 N/A
della global (gai-t6/ rga-t2/ rgl1-1/ rgl2-1/ rgl3-4) 49 N/A
pPIL1:LUC 17 N/A
pIAA19:LUC New line N/A
pDR5V2:GUS New line N/A
pDR5V2:GUS/ pif4-101/ pif5 (pil6-1) New line N/A /Garlic_114_G06/ SALK_087012
p35S:PIF4-HA Franklin Lab, Bristol, UK N/A
p35S:PIF5-HA Franklin Lab, Bristol, UK N/A
p35S:BES1-GFP 28 N/A
Tomato- S.lycopersicum var. “Moneymaker” Intratuin, NL EAN# 8717263349600
Tobacco- N. benthamiana N/A N/A

Recombinant DNA

pENTR_D-TOPO Thermo Fisher K2400-20
LucTrap3 N/A GenBank: AY968054.1
pGREENII0179 N/A N/A
pUC57:DR5v2 Dolf Weijers (WUR) N/A

Software and Algorithms

Microsoft Excel 2010 Microsoft N/A
Microsoft Powerpoint 2010 Microsoft N/A
Microsoft Word 2010 Microsoft N/A
SPSS Statistics 24 IBM N/A
Snapgene GSL Biotech LLC N/A
ImageJ NIH N/A
ICY Quantitative Image Analysis Unit, Institut Pasteur N/A
GIMP2 The GIMP Development Team N/A
ViiA 7 Software Thermo Fisher Scientific N/A
Prism 6 Graphpad N/A