Figure 1.

Chronic nicotine exposure alters spontaneous action potential firing in MHb neurons. a, Representative cell-attached firing traces for MHb neurons from control and cNIC-treated mice. b, Summary data (control: n = 11 cells, n = 3 male mice; cNIC-treated: n = 10 cells, n = 4 male mice) of cell-attached firing in MHb neurons for control and cNIC-treated mice. c, Representative spontaneous action potentials for whole-cell patch-clamped MHb neurons for control and cNIC-treated mice, illustrating features quantified in subsequent panels. d, Representative spontaneous action potential phase plots for MHb neurons from control and cNIC-treated mice. e, Summary resting membrane potential data (control: n = 34 cells, n = 5 male mice; cNIC-treated: n = 31 cells, n = 7 male mice; the same mice were used for data in f–j) for MHb neurons from control and cNIC-treated mice. f, Summary action potential amplitude data (control: n = 32 cells; cNIC-treated: n = 30 cells) for MHb neurons from control and cNIC-treated mice. g, Summary action half-width data (control: n = 32 cells; cNIC-treated: n = 30 cells) for MHb neurons from control and cNIC-treated mice. h, Summary action potential threshold data (control: n = 32 cells; cNIC-treated: n = 29 cells) for MHb neurons from control and cNIC-treated mice. i, Summary action potential maximum rise slope data (control: n = 32 cells; cNIC-treated: n = 31 cells) for MHb neurons from control and cNIC-treated mice. j, Summary action potential maximum decay slope data (control: n = 32 cells; nicotine-treated: n = 31 cells) for MHb neurons from control and cNIC-treated mice.