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. 2019 Jun;369(3):481–488. doi: 10.1124/jpet.118.254201

Fig. 3.

Fig. 3.

Transcytosis of AF647-ApoA-I across hCMEC/D3 monolayers cultured on 0.4 µm Transwell filters. (A) The Z-stack composite image demonstrates internalization of AF647-ApoA-I (0.4 µM) in polarized hCMEC/D3 monolayers after 1 hour of incubation on the luminal side. The image presented in X-Y (transversal), X-Z (vertical), and Y-Z (vertical) planes is a composite of 32 optical sections imaged with a 0.53 µm Z-step interval. Red = AF647-ApoA-I; blue = DAPI-stained nuclei. (B) Enlarged section of the Y-Z plane showing permeation of AF647-ApoA-I across the endothelium. (C) Western blot showing ApoA-I in hCMEC/D3 lysate after 1 hour of incubation on the luminal side. (D) Western blot showing ApoA-I on the abluminal side after 1 hour of incubation on the luminal side.