Fig. 4.

NL-1 decreases cell viability in ALL cell lines. ALL cell lines, (A) REH, REH/Ara-C and (C) NALM6, NALM1, SUPB15, TOM1, BV173, and JM1, were plated at 5 × 10⁴ cells/well in a 96-well cell culture plate and treated with indicated concentrations of NL-1. The number of live cells was measured after 72 hours of treatment (B and D). IC₅₀ values were calculated from the concentration-dependent curves of each cell line using the CompuSyn software. The data are represented as mean ± S.E.M. of a study performed in triplicate and are representative of experiments that were carried out at least three independent times.