Table 1.

Low Off-Target Activity of IL2RG CRISPR/Cas9 System in Human NSCs

	Unselected	CD8 Selected	CD8 Selected	tCD19 Selected
IL2RG	28.35	75.85	59.11	71.27
Off-target 1	0	0	0.01	0
Off-target 2	0	0.28	0.07	0
Off-target 3	0	0	0	0.62

Targeted deep sequencing of IL2RG alleles and three previously predicted off-target sites was performed on unedited NSCs and GE-NSCs. Each column represents an independent targeting experiment from GE-NSCs that were unselected, CD8 selected, or tCD19 selected. Off-targets were previously predicted using bioinformatic tools (Hendel et al., 2015). All numbers are subtracted from background INDEL frequencies in an unedited NSC sample.