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. Author manuscript; available in PMC: 2019 May 29.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Sep 6;414(1):25–30. doi: 10.1016/j.bbrc.2011.09.011

Fig. 2.

Fig. 2

shRNA silencing of individual HDACs increase SMN-luciferase activity and mRNA. (A) HDAC shRNAs increase SMN-luciferase activity levels. SMN2 reporter cells were treated for 48 h with shRNA. HDAC2 silencing increase luciferase activity the greatest at nearly 2-fold. HDAC5 knockdown appears to have no effect on luciferase activity (B) qRT-PCR was used to measure increases of SMN-luciferase mRNA following treatment with HDAC shRNA. Fold increase of mRNA was normalized to GAPDH.