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. Author manuscript; available in PMC: 2019 May 29.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Sep 6;414(1):25–30. doi: 10.1016/j.bbrc.2011.09.011

Fig. 3.

Fig. 3

Quantitative PCR of endogenous splicing factors in response to HDAC inhibitor treatment in SMN2-luciferase reporter cells. SMN2-luciferase reporter cells were treated with 4 concentrations of HDAC inhibitors SAHA, M344, and MS-275. mRNA levels of splicing factors hTra2b (white bars), hnRNPA1 (gray bars), and SF2/ASF (dark gray bars). qRT-PCR was used to measure fold changes in transcript levels relative to DMSO carrier treated cells. GAPDH was used for normalization. (B) SMN2-luciferase reporter cells were treated with HDAC specific shRNA. mRNA levels of splicing factors hTra2b (white bars), hnRNPA1 (gray bars), and SF2/ASF (dark gray bars). qRT-PCR was used to measure fold changes in transcript levels relative to a non-silencing shRNA control. GAPDH was used for normalization.