Figure 4.

Meta-analysis of T1R3 TMD site-directed mutagenesis data. Experimental data have been summarized from Jiang et al. (2005b) and Winnig et al. (2007), represented by triangle and square symbols. New site-directed mutagenesis experiments reported in the present study are indicated by filled circles. Receptor responses indicate the effect of mutations on the receptor activity, expressed as a percentage compared to the wild-type receptor, to the allosteric (cyclamate in x axis) and orthosteric (d-trp and aspartame in y axis) ligands. Cyclamate-specific interacting residues are in blue. Residues that control the signaling pathway or abolish the receptor response are colored in green and red, respectively. Residues that have no or weak-specific effect on receptor response are in black. All data are summarized in Supplementary Table 3.