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. 2019 Apr 24;44(5):289–301. doi: 10.1093/chemse/bjz019

Figure 2.

Figure 2.

Expression of GFP and M71 olfactory receptors in transgenic mice taste tissue is not an artifact or genetic alteration. The expression of GFP and M71 olfactory receptors in transgenic and wild-type (C57BL/6) mice was visualized using GFP and M71 antibodies. (A) and (C): Transmission images of corresponding fields from taste papillae of wild-type mouse. (B): No immunoreactivity (absence of red) to GFP antibody in circumvallate taste tissue of wild-type (C57BL/6) mouse. (D): Immunoreactivity to M71 olfactory receptor antibodies (red) in the taste papillae of wild-type mouse. (E–H): Immunostaining of mOR-EG-GFP mouse taste papilla tissue with GFP antibody (G, red) shows exact colocalization with endogenous GFP expression (F, green; H, merged). (I–L): Taste papilla tissues from mOR-M71-GFP mice demonstrate expression of GFP (green, J) and M71 (red, K) is colocalized (L, merged). E and I are transmission images of corresponding fields in their respective rows. Blue, nuclei staining with DAPI. Scale bars = 25 µm.