Table 2.

Plackett Burman experimental design matrix for screening medium components of act-D production.

Trial	Coded variables						Actinomycin D (mgL−1)
	x1	x2	x3	x4	x5	x6
1	-	+	-	-	-	+	191.59 ± 1.41
2	-	+	+	+	-	+	278.85 ± 14.14
3	-	-	-	+	+	+	290.26 ± 5.64
4	+	+	+	-	+	+	153.28 ± 9.89
5	-	+	+	-	+	-	206.87 ± 9.88
6	+	-	+	-	-	-	180.31 ± 11.31
7	+	-	-	-	+	+	163.68 ± 8.48
8	-	-	+	+	+	-	168.88 ± 7.07
9	+	-	+	+	-	+	122.92 ± 2.82
10	-	-	-	-	-	-	479.74 ± 9.89
11	+	+	-	+	-	-	154.82 ± 4.24
12	+	+	-	+	+	-	103.17 ± 8.48