Figure 1.

Exposure to hypoxia (1% oxygen) enhances metabolism but not proliferation. Glioblastoma cell lines- U87, U251 andSNB19, were setup with seeding density of 2000 cells per well in a 96-well plate. On the same day of setup, a day 0 reading was taken after cells had settled and a set of cells was transferred to hypoxia (un-dotted), while the control cells were maintained in normoxia (dotted). Metabolism was assessed with AlamarBlue (A–C), while proliferation was measured with the CyQUANT cell assay (D–F). The cells were read at the indicated times. The error bar represents standard error from 3 independent experiments for U87, U251, and n = 2 for SNB19 cells. Student’s t-test from Prism7 was used for statistical comparison. * p  <  0.05, ** p  <  0.01.