Figure 5.

CD133 protein is upregulated under hypoxic conditions. U87 (A–C), and U251 (E–H) cells were cultured under normoxic (A,B,E,F) and hypoxic (C,D,G,H) conditions for 72 h. For both conditions, the total isotype control cell populations (A,C,E,G) are presented based on side and scatter properties, and appropriate regions are gated and used to compare cells stained with the anti-CD133 antibody (B,D,F,H). The percentages of cells expressing CD133 after 72 h are indicated. The analyses were performed using Weasel software. The results are representative of at least 3 independent experiments.