Figure A2.

Agarose gel electrophoreses of products generated by the last round of 5’RACE (rapid amplification of cDNA 5′-end) in two experiments. (a) The third round of classic 5’RACE. RNA samples from two snails (sn1 and sn2) were initially used, but RACE products were later purified and cloned only for snail 1. (b) The second round of RLM-5’RACE (RNA ligase-mediated rapid amplification of cDNA 5′-end), snail 3 (sn3). (c) The second round of RLM-5’RACE, snail 4 (sn4). M1, M2—molecular weight markers (Thermo Scientific #SM0311 and Thermo Scientific #SM1331, respectively); white numbers represent marker bars lengths (bp).