Table 2.
Gene-specific primers designed to assess expression levels of aPKC mRNA isoforms.
| Primer Target | Primer Name | Primer Sequence (5′→3′) |
|---|---|---|
| aPKC X1 mRNA isoform | aPKC X1 F | CTGCATGTGCATTTTGCC |
| aPKC X1 R | TTTCACATCCTCCAGTGTTCC | |
| aPKCΔC1 mRNA isoform (primers for qPCR) | aPKCΔC1 F | TATAGGAGAGGGGCTCG |
| aPKCΔC1 R | ATGTTCTTTCACATCCCTTG | |
| aPKCΔC1 mRNA isoform (primers for ddPCR) | dd aPKCΔC1 F | GCCAAGAGATTTTCAAGGGAT |
| dd aPKCΔC1 R | CTCCATTCACAGGTTGCG | |
| Putative kinase domain-coding part of aPKC mRNA | aPKC KD F | TGAGTTTGTGAATGGAGGCG |
| aPKC KD R | AGTCTGTTAGTTTGATGTGTCCC | |
| TATA-box binding protein | TBP F | GGTTGGTAGCTGTGATGTC |
| TBP R | CCATGCGGTAGATAAGTCC | |
| Glyceraldehyde 3-phosphate dehydrogenase | GAPDH F | CCCAGAACATCATTCCCTCCTC |
| GAPDH R | CGGAAAGCCATGCCGGT |