Table 2.
Experimental design.
| Run | Coded Level of Variable | Reducing Sugar | DP f | |||||
|---|---|---|---|---|---|---|---|---|
| T a | pH | E/S b | T c | C.j. d | C.t. e | C.j. d | C.t. e | |
| 1 | −1 | −1 | −1 | −1 | 0.2048 | 0.1427 | 2.7 | 4.1 |
| 2 | 2 | 0 | 0 | 0 | 0.0383 | 0.1421 | 14.2 | 4.1 |
| 3 | 0 | 0 | 0 | 0 | 0.2131 | 0.1166 | 2.6 | 5.0 |
| 4 | 0 | 0 | 0 | 0 | 0.2670 | 0.1205 | 2.0 | 4.9 |
| 5 | 1 | −1 | −1 | −1 | 0.2509 | 0.1599 | 2.2 | 3.7 |
| 6 | 1 | 1 | −1 | −1 | 0.0522 | 0.0883 | 10.4 | 6.6 |
| 7 | 1 | −1 | −1 | 1 | 0.2359 | 0.1998 | 2.3 | 2.9 |
| 8 | −1 | −1 | 1 | −1 | 0.2392 | 0.146 | 2.3 | 4.0 |
| 9 | −1 | −1 | 1 | 1 | 0.2864 | 0.1743 | 1.9 | 3.4 |
| 10 | 1 | 1 | 1 | 1 | 0.1371 | 0.1349 | 4.0 | 4.3 |
| 11 | 0 | 2 | 0 | 0 | 0.1726 | 0.1132 | 3.2 | 5.2 |
| 12 | 0 | 0 | 2 | 0 | 0.2792 | 0.1682 | 2.0 | 3.5 |
| 13–16 | 0 | 0 | 0 | 0 | 0.2409 | 0.1427 | 2.3 | 4.1 |
| 17 | −1 | 1 | −1 | −1 | 0.1349 | 0.0644 | 4.0 | 9.1 |
| 18 | −1 | 1 | 1 | 1 | 0.2243 | 0.1277 | 2.4 | 4.6 |
| 19 | −1 | −1 | −1 | 1 | 0.2548 | 0.0816 | 2.1 | 7.2 |
| 20 | 0 | 0 | −2 | 0 | 0.1837 | 0.0788 | 3.0 | 7.4 |
| 21 | 0 | 0 | 0 | 2 | 0.2537 | 0.1482 | 2.1 | 4.0 |
| 22 | 0 | 0 | 0 | −2 | 0.1993 | 0.101 | 2.7 | 5.8 |
| 23 | 1 | 1 | 1 | −1 | 0.1282 | 0.1299 | 4.2 | 4.5 |
| 24 | 0 | 0 | 0 | 0 | 0.2720 | 0.1610 | 2.0 | 3.6 |
| 25 | −1 | 1 | −1 | 1 | 0.1965 | 0.086 | 2.8 | 6.8 |
| 26 | 0 | −2 | 0 | 0 | 0.2415 | 0.0955 | 2.3 | 6.1 |
| 27 | 1 | 1 | −1 | 1 | 0.0866 | 0.1138 | 6.3 | 5.2 |
| 28 | −2 | 0 | 0 | 0 | 0.2342 | 0.1044 | 2.3 | 5.6 |
| 29 | 1 | −1 | 1 | −1 | 0.2737 | 0.1843 | 2.0 | 3.2 |
| 30 | 1 | −1 | 1 | 1 | 0.2964 | 0.232 | 1.8 | 2.5 |
| 31 | −1 | 1 | 1 | −1 | 0.2065 | 0.0822 | 2.6 | 7.1 |
a incubation temperature (°C); b enzyme/substrate ratio (U/mg); c incubation time (min); d galactanase from C. japonicus (C.j.), total sugar = 0.5447967 μmol; e galactanase from C. thermocellum (C.t.), total sugar = 0.58664211 μmol; f degree of polymerization (DP) estimated by the ratio total sugar/reducing sugar.