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. 2019 May 7;24(9):1762. doi: 10.3390/molecules24091762

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(a) Live/dead staining of mono-cultured 3D lobule-like microtissues and co-cultured 3D microtissues after 7 days of culture, scale bar: 200 μm; (b) The cell viability of mono-cultured 3D microtissues which encapsulating HepG2 cells and co-cultured 3D microtissues which encapsulating HepG2 cells and NIH/3T3 cells; (c) The evaluation of albumin secretion of HepG2 cells in mono-cultured 3D microtissues and co-cultured 3D microtissues during long-term culture; (d) The evaluation of urea synthesis of HepG2 cells in mono-cultured 3D microtissues and co-cultured 3D microtissues during culture.