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. 2019 May 24;11:4781–4796. doi: 10.2147/CMAR.S207257

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© 2019 Fei et al.

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Eupatilin affects protein expression in EMT and RECK/MMP pathways. (A) Cell membrane of U251MG was stained with DiI (20 μM) for 30 mins after 24 hrs of treatment with eupatilin (wavelength: 555 nm). (B) Western blot detects the expression of related proteins in EMT and RECK/MMP pathways. GAPDH is used as a housekeeping protein to prove the equal loading in each lane of the electrophoresis and to normalize densitometric values of the other protein analyzed. Each experiment was repeated three times.