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. 2019 May 3;20(9):2190. doi: 10.3390/ijms20092190

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of silybin on cell cycle. (up) Cell cycle distribution after 72 h of treatment with silybin IC50 (68 μM) in HepG2 cells, evaluated by FACS, as described in Materials and Methods. Data are expressed as mean ± SD of the percentage of cells in the different phases of the cell cycle, as compared with untreated control cells. (down) Effects of silybin on p21 and cyclin A expression in HepG2 cells. γ-tubulin was used as loading control. The intensity of each band was expressed as % arbitrary units compared to that of the untreated cells. Error bars showed standard deviation from the mean in at least three independent experiments.