Table 7.
A summary of the heterologous overexpression screenings performed in yeast using plant cDNA libraries.
| Plant Species | cDNA Library [Reference] | Yeast Strain | Screening Conditions | Isolated Genes | Reference |
|---|---|---|---|---|---|
| Arabidopsis thaliana | Leaves from Arabidopsis seedlings [239] | ena1-4 (W303-1A) | 50 mM LiCl | AtRCY1 (K/T-cyclin with SR domain of splicing proteins) AtSRL1 (SR domain of splicing proteins) |
[237] |
| AtLTL1 (GDSL-motif lipase) | [240] | ||||
| [241] | cna1cna2YPH499 | 200 mM NaCl | AtSTZ1 (Zinc finger protein) AtSTO1 (B-Box domain protein 24) |
[242] | |
| Atriplex canescens | Young leaves and stems treated with 400 mM NaCl [243] | WT (INVSc1) | 2 M NaCl |
KJ026992 (Cyclophilin) KJ027014 (Glycine-rich protein) KJ027023 (Cytochrome P450) KJ027035 (Temperature-induced lipocalin) KJ027049 (Cysteine proteinase A494) KJ027057 (Alanine aminotransferase 2) KJ027061 (Hexose transporter) KJ027088 (RNA-binding family protein) KJ027102 (Cysteine proteinases) KJ027110 (calmodulin1) |
[244] |
| Beta vulgaris | Leafs from salt stressed plants [245] | ena1-4 nha1(W303-1A) | 150 mM NaCl | BvCK2 (catalytic subunit of the casein kinase) | [245] |
| BveIF1A (Translation initiation factor) | [246] | ||||
| BvSATO1 (RNA binding protein with RGG and RE/D motifs) BvSATO2 (homologous to SATO1) BvSATO4 (RNA binding protein) BvSATO5 (RNA binding protein) BvU2AF (U2snRNP AF protein) |
[247] | ||||
| gpd1(W303-1A) | 1 M Sorbitol | BvSAT1 (Serine acetyl trasferase 1) | [248] | ||
| BvGLB2 (Type II non symbiotic plant hemoglobin) | [249] | ||||
| WT (W303-1A) | 10 °C | BvCOLD1 (TIP-like aquaporin) | [250] | ||
| Ipomoea pes-caprae | Growing leaves, shoots and roots [251] | ena1-4 nha1 nhx1(W303-1A) | 75 mM NaCl |
MF680587 (putative abscisic acid, stress, and ripening-induced protein (ASR)) MF680589 (nudix hydrolase, chloroplastic) MF680592 (F-box protein At5g46170-like) MF680597 (fructokinase) MF680602 (adenosylhomocysteinase 1) MF680603 (peptide upstream protein) MF680604 (catalase) MF680608 (40S ribosomal protein S25) MF680611 (phosphomannomutase) MF765747 (dnaj protein-like protein) MF680614 (phytosulfokines-like) MF680616 (sugar carrier protein C) MF680619 (abscisic acid 8′-hydroxylase 4) KX426069 (dehydrin) |
[251] |
| Jatropha curcas | 3-4 week seedlings treated with 150 mM NaCl [252] | shs-2 (UV-generated mutant in the BY4741 background) | 750 mM NaCl |
FJ489601 (Allene oxide cyclase) FJ489602 (Thioredoxin H-type (TRX-h)) FJ489603 (Metallothionein) FJ489604 (Heterotrophic ferredoxin) FJ489605 (Defensin) FJ489606 (Calmodulin-7 (CAM-7)) FJ489608 (S18.A ribosomal protein) FJ489609 (60S ribosomal protein L18a) FJ489611 (Unknown protein) FJ619041 (Membrane protein -2) FJ619045 (Profilin-like protein) FJ619048 (Copper chaperone) FJ619052 (Annexin-like protein) FJ619053 (Al-induced protein) FJ619055 (60S ribosomal protein L39) FJ619056 (Ribosomal protein L37) FJ619057 (Ribosomal protein L15) FJ623457 (40S ribosomal protein S15) FJ623458 (40S ribosomal S18) |
[252] |
| Oryza sativa | Leaves from seedlings treated with different abiotic stresses [253] | WT (AH109) | 900 mM NaCl | OsMPG1 (mannose-1-phosphate guanyl transferase gene) | [253] |
| Paspalum vaginatum | Cultivated stolons treated with 250 mM NaCl [254] | ena1-4(G19) | 500 mM NaCl |
KT203435 (Uncharacterized protein) KT203436 (Iron-regulated transporter) KT203439 (Early light-induced protein) KT203440 (14-3-3-Like protein) KT203441 (Class 1 HSP) KT203442 (Cysteine synthase) KT203443 (Aldo-ketoreductase) KT203444 (L-Ascorbate peroxidase 2) KT203447 (Nop14-like family protein) KT203450 (Protein IQ-DOMAIN 14-like) KT203451 (Metacaspase-5-like) |
[254] |
| Phoenix datilifera | Salt-treated roots [255] | WT (INvSc1) | 2 M NaCl |
XM_008806660.2 (11S globulin seed storage protein 2-like) XM_008805834.2 (ABC transporter) XM_008780694.2 (Aquaporin PIP1-2) XM_008793314.1 (Aquaporin PIP2-4-like) XM_008779330.1 (Cysteine desulfurase) XM_008802947.2 (Cytochrome b5-like) XM_008783561.2 (Cytospin-A-like 1) XM_008797620.2 (Hexokinase-2-like) XM_008780092.2 (Mavicyanin-like 1) XM_008814440.2 (Peroxidase 3-like) XM_008786338.2 (Peroxidase 3-like 2) XR_604439.2 (Uncharacterized) XM_008800513.2 (Uncharacterized) XM_017846106.1 (Uncharacterized) XM_017846788.1 (Uncharacterized) |
[255] |
| Salicornia europaea | 3-month-old plants | WT (BY4741) | 1.6 M NaCl | SeNN8 (Similar to FKBP5) SeNN24 (Thaumatin like protein) SeNN43 (Unknown protein) |
[256] |
| Solanum tuberosum | Plants grown in vitro and subjected to heat shock at 35 °C [257] | WT (BY4741) | 39 °C | StnsLTP1 (Non-specific Lipid Transfer Protein-1) | [258] |
| Zea mays | Maize kernels | Not indicated | Not indicated | MBF1a (Multiprotein bridging factor 1a transcriptional coactivator) | [259] |
| Zoysia matrella | Cultivated stolons treated with 300 mM NaCl | ena1-4 (G19) | 500 mM NaCl |
KM265171 (Uncharacterized protein) KM265174 (C2H2-type Zinc finger protein) KM265176 (Unknown protein) KM265177 (Alcohol dehydrogenase 1) KM265179 (Protein disulfide isomerase) KM265182 (Glyoxylate reductase) KM265183 (Serine carboxypeptidase) |
[260] |