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. 2019 May 22;10:584. doi: 10.3389/fphar.2019.00584

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Copyright © 2019 Ouyang, Huang, Liu, Wu, Liu and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RbCl attenuates osteoclast formation and function without causing cytotoxicity in vitro. (A) Cell viability of osteoclast precursors after RbCl treatment from 24 to 96 h. (B) In vitro osteoclast formation of primary BMMs and RAW264.7 cells after RANKL and RbCl treatment. (C) Quantification of osteoclastogenesis by RbCl. (D) Formation of F-actin ring and bone resorption pits after RANKL and RbCl treatment. (E) Quantification of F-actin ring and bone resorption pits. *p < 0.05 compared with control. Each experiment was repeated biologically in triplicate independently.