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. 2019 Apr 27;20(9):2079. doi: 10.3390/ijms20092079

Figure 1.

Figure 1

Origin of the different callus types for Tuxpeño VS-535. (A) Immature embryo (IE) at 15–18 days after pollination (DAP). (B) The IE observed with SEM. The embryonic axis and surrounding tissue are clearly distinguished. (C) The IE after seven days on N6I medium. Radicle protrusion and callus formation around the embryo are observed. (D) The IE after seven days on N6I medium observed with SEM. (E) Magnification of the circled area in D. The arrow points at non-embryogenic callus formation, whereas the arrowhead indicates pro-embryogenic callus tissue. (F) The IE after nine days on N6I medium observed with SEM. Cumulus of embryogenic masses formed on the scutellar epithelium are highlighted by several arrowheads, whereas an arrow points at non-embryogenic callus formed on the embryonic axis side. (G) Magnification of F in an arrowhead zone, (H) Magnification of G in the arrow zone.