Table 4.
Primer Sequences for qRT-PCR
| Gene | Sequence | |
|---|---|---|
| miR-133b | forward | 5′-TTGGTCCCCTTCAACCAGCTA-3′ |
| reverse | 5′-CAGTGCGTGTCGTGGAGT-3′ | |
| U6 | forward | 5′-CTCGCTTCGGCAGCACA-3′ |
| reverse | 5′-ACGCTTCACGAATTTGCGT-3′ | |
| Notch1 | forward | 5′-GATGGCCTCAATGGGTACAAG-3′ |
| reverse | 5′-TCGTTGTTGTTGATGTCACAGT-3′ | |
| Jagged-1 | forward | 5′-ATGCAGAACGTGAATGGAGAG-3′ |
| reverse | 5′-GCGGGACTGATACTCCTTGAG-3′ | |
| DLL4 | forward | 5′-TTCCAGGCAACCTTCTCCGA-3′ |
| reverse | 5′-ACTGCCGCTATTCTTGTCCC-3′ | |
| Bax | forward | 5′-GTTTCATCCAGGATCGAGCAG-3′ |
| reverse | 5′-CCCCAGTTGAAGTTGCCATC-3′ | |
| Bcl-2 | forward | 5′-GGTACCGGAGAGCGTTCAGT-3′ |
| reverse | 5′-CTGCTGCATTGTTCCCGTAG-3′ | |
| Caspase-3 | forward | 5′-AGCTTCTTCAGAGGCGACTA-3′ |
| reverse | 5′-GGACACAATACACGGGATCT-3′ | |
| MAML1 | forward | 5′-CTGCCTCCCCTCAGAATGG-3′ |
| reverse | 5′-CTCCCGGCGTGTCTTCTTAT-3′ | |
| GAPDH | forward | 5′-GGGAAATTCAACGGCACAGT-3′ |
| reverse | 5′-AGATGGTGATGGGCTTCCC-3′ | |
miR-133b, microRNA-133b; DLL4, Delta-like ligand 4; Bcl-2, B cell lymphoma-2; Bax, Bcl2-associated X protein; MAML1, mastermind-like 1, GAPDH, glyceraldehyde-3-phosphate dehydrogenase.