Table A1.
The primers used in this study.
| Primer | Sequence (5′ to 3′) | Note |
|---|---|---|
| ANS-F1 | CGTGATGCACCTGGTCaaycayggnrt | Homologous cloning of SmANS |
| ANS-R2 | GGACAGGATCTCGATGGTGtcnccdatrtg | |
| ANS-R3 | CAGGCCGGGCaccatrttrtg | |
| SmANS-F1 | CTGAAGCGGGAAGGGACT | Full length cDNA cloning of SmANS |
| SmANS-R1 | GAGGACCTAACAGAACTCGCC | |
| SmANS-GFPf | TTTTTTGTCGACATGGTTGCTACACCGG (SalI site underlined) |
Construction of subcellular localization vector |
| SmANS-GFPr | GGGACTAGTGCACTCGATTTATCATAATCAGGC (SpeI site underlined) |
|
| SmANS-OE-F | TTTTGGATCCATGGTTGCTACACCGG (BamHI site underlined) |
Construction of overexpression vector |
| SmANS-OE-R | CGCGGGGTACCTCAACTCGATTTATCA (KpnI site underlined) |
|
| 2300NPTII-F | ATACCGTAAAGCACGAGGAAG | Transgenic lines identification |
| 2300NPTII-R | CTGAAGCGGGAAGGGACT | |
| CaMV35S-F | GAGGACCTAACAGAACTCGCC | |
| SmANS-O-R | CCAAATAGACAAGTCCCTCT | |
| SmCHI-F | TTCCATTCCAGAGCAAGGCG | qRT-PCR analysis |
| SmCHI-R | CCTTGCAGTAGGCGACACTCCTT | |
| SmUF3GT-F | AGCCAAAACCGCCCTAATTCAGTA | |
| SmUF3GT-R | ATGGGAATCCGCATGTTTCTAGG | |
| SmFSNII-F | TCGACCCGATCATCACCAAAGAC | |
| SmFSNII-R | GGGAATCCGCATGTTTCTAGG | |
| SmDFR-F | TAAACTTCATCAGCATCATACCACC | |
| SmDFR-R | TCCTTGCTTTATGATGGAGTAGTG | |
| SmANS-F | CTTGTCTATTTGGCCCAAGCACC | |
| SmANS-R | CTGAGGGCATTTCGGGTAGAAG | |
| SmF3H-F | AAAGCGTGCGTTGATATGG | |
| SmF3H-R | GATCCATGTATTACCACCATCC | |
| SmF3’H-F | TGCCACGAACGCAATAGCTC | |
| SmF3’H-R | TTGAATACTCCAGCCAACGACA | |
| SmActin-F | GGTGCCCTGAGGTCCTGTT | |
| SmActin-R | AGGAACCACCGATCCAGACA |