Figure 2.

Potent and Durable C5 Silencing and Complement Activity Reduction in NHPs with Single-Dose ALN-CC5 Treatment

(A) C5 protein quantified by ELISA levels following a single s.c. injection of ALN-CC5. N = 6 through day 36, N = 4 on day 43, and N = 2 on day 71. For 0.2–5 mg/kg treatments, C5 levels were normalized to the average C5 levels at 2 and 15 min and 8 h after dose time points, which were expected to be equivalent to baseline levels. For the 25 mg/kg group, C5 levels are normalized to the level at 24 h after the dose, expected to be within 20% of baseline. (B) Hemolytic complement activity levels were evaluated in serum samples collected as in (A), using a sheep RBC hemolysis assay. Hemolysis values were normalized to a “maximal hemolysis” control (lysis by water). Group averages with SD are plotted for both readouts.