Figure 7.

C5 siRNA Treatment Protects the Neuromuscular Junctions of EAMG Rats from Complement-Mediated Destruction in Both Pre- and Post-Treatment Models

Tibialis anterior muscle sections were stained with Alexa-594-labeled bungarotoxin for identification of NMJ, Alexa-488-labeled anti-rabbit IgG for detection of MAC deposition, and Alexa-488-labeled anti-mouse IgG for detection of C3. Immunofluorescence confocal microscopy was performed. Quantitation of fluorescence intensity was performed using Zen software. Mean pixel intensity values were plotted for each group (n = 8 per group). Statistical significance was obtained by t test and p < 0.05 was considered as significant. Error bars are SD. (A) Mean fluorescence intensity of bungarotoxin at the NMJ. (B) Mean fluorescence intensity of MAC deposition at the NMJ. (C) C3 deposition at the NMJ.