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. 2019 Apr 28;20(9):2095. doi: 10.3390/ijms20092095

Figure 3.

Figure 3

Cross-interaction between BCCIP, YY1, and p53 was verified in HCT116 (p53+/+) cells. (A) Interaction between BCCIP and YY1. In vitro co-transfection/coimmunoprecipitation (CoIP) experiments with anti-Flag M2 and anti-c-Myc affinity agarose were carried out in HCT116 (p53+/+) and HCT116 (p53−/−) whole-cell lysates. Bound proteins were visualized by western blot. (B) Interaction between p53 and BCCIP in shNT- and shYY1-treated HCT116 cells. CoIP experiments with anti-Flag M2 and anti-Myc antibodies were performed in HCT116 cells, and bound proteins were detected by western blot. (C) Interaction between p53 and YY1 in shNT- and shBCCIP-treated HCT116 cells. CoIP experiments with anti-Flag M2 and anti-Myc antibodies were done as designed.