Figure 6.

P53 downstream target gene p21 was coordinately regulated by YY1 and BCCIP in HCT116 (p53+/+) cells. (A) Induction of p53 by 250 µM 5FU. Indicated proteins were analyzed by western blot with specific antibodies. (B) Impact of shBCCIP on 5FU-induced p53. shNT- and shBCCIP-transfected cells were treated with 0 and 250 µM 5FU, and indicated proteins were measured by western blot with antibodies. (C) Coordinative effects of YY1 and BCCIP on p53. The experiment was designed as shown. Proteins in prepared lysate were analyzed by western blot. (D) Immune staining. Cells were transfected with indicated plasmids in the presence or absence of 5FU. Then, cells were stained with anti-p21 antibody. DAPI staining shows total nuclei. Scale bar indicates 200 µm.