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. 2019 Apr 28;20(9):2095. doi: 10.3390/ijms20092095

Figure 8.

Figure 8

YY1 and BCCIP coregulation of p53 target gene p21 in shBCCIP-treated HCT116 cells. BCCIP knockdown cells with or without 5FU exposure were used in the following ChIP experiments. (A,B) p53 and YY1 ChIP experiments were carried out using chromatin lysate in the presence of shNT or shBCCIP expressing cells. Chip DNA was analyzed with qPCR (n = 3). (C,D) Rescue experiments. Stably expressing shNT or shBCCIP cells were transiently transfected with Flag-YY1 for 48 h. Then, prepared chromatin lysate was applied to ChIP assays. The y-axis shows the ratio of ChIP DNA signals to IgG, and all signals were normalized to input (n = 3). * p < 0.05, ** p < 0.01, t-test, comparison to shNT group. (E) Indicated protein levels in A, B, C and D.