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. 2019 Apr 30;20(9):2135. doi: 10.3390/ijms20092135

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of TGFβ, TNFα, or concomitant TGFβ + TNFα treatments on vector-only and HDAC5 expressing UCCs. Cells were treated with TGFβ, TNFα, or both, or left untreated. Cell proliferation was followed by MTT assays at 24, 48, 72, and 96 h. The 24 h data of each cell line was set as “1”. Values represent means ± SD (error bars) of quadruplicates. left: RT112, right: VM-Cub-1 cells. Black lines and symbols: vector-only cells; Blue lines and symbols: HDAC5 transduced cells. All cytokine treatments caused significant (t-test, p < 0.05) decreases compared to the respective untreated controls, except for the TGFβ treatment of RT112 at 96 h.