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. 2019 May 2;20(9):2177. doi: 10.3390/ijms20092177

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Translation of genes modulated in high-grade fragments are not dependent on mTORC1. U-87MG GBM cells were serum starved (Control), treated with serum (FCS) and serum plus the mTOR inhibitor Torin1. (A) Phosphorylation of mTORC1 targets (p70S6K and 4E-BP) was analyzed by Western blot as readout for mTORC1 activity. Polysome profiles were performed (B) and RNA was extracted and used for (C) qPCR for genes regulated by translation (LOX, WISP), abundance (FOXC1, COL5A2, ITGA11), buffering (SNAI2), or whose translation is regulated by mTORC1 (NAP1L1, RPL13, RPL35 and AP2A). Graph displays the polysome/total ratio of expression for each mRNA. * p < 0.05, One Way ANOVA followed by Tukey’s t test.